ANNE, Agnès; CHOVIN, Arnaud; DEMAILLE, Christophe; MICHON, Thierry

doi:10.1007/978-1-4939-7808-3_30

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Licencia de uso del documento

ANNE, Agnès
Université Paris Diderot - Paris 7 [UPD7]

CHOVIN, Arnaud
Université Paris Diderot - Paris 7 [UPD7]

DEMAILLE, Christophe
Université Paris Diderot - Paris 7 [UPD7]

Idioma

Chapitre d'ouvrage

Este ítem está publicado en

Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols, Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols. 2018, vol. 1776

Humana Press

Resumen en inglés

We present in this chapter a new experimental approach allowing the high resolution imaging of immune complexes on virus particles. Combined atomic force-electrochemical microscopy (AFM-SECM) is used to image the presence of ferrocene functionalized specific antibodies on the surface of potyvirus particles. For this purpose, potyviruses, flexuous filamentous phytoviruses with a high aspect ratio, have been chosen. This technique allows analysis of the distribution of antibody labeling over the virus population. But, more importantly, it opens up the imaging of immune complexes decorating a single viral particle. Finally, its high resolution allows the characterization in situ of the ultrastructure of a single immune complex on the particle.< Leer menos

Palabras clave

Electrochemical atomic force microscopy

Lettuce mosaic virus (LMV)

Palabras clave en inglés

Potyvirus

Redox antibodies

Single viral protein imaging

Viral nanoparticles

DOI

http://dx.doi.org/10.1007/978-1-4939-7808-3_30

Proyecto ANR

Imagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009

Orígen

Importado de Hal

Centros de investigación

Biologie du Fruit & Pathologie (BFP) - UMR 1332