Analysis of enzyme activities
Idioma
en
Chapitre d'ouvrage
Este ítem está publicado en
Plant Metabolic Flux Analysis : Methods and Protocols, Plant Metabolic Flux Analysis : Methods and Protocols. 2014, vol. 1090
Springer Science+Business Media
Resumen en inglés
The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the ...Leer más >
The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented.< Leer menos
Palabras clave
microplate
Palabras clave en inglés
enzyme activity
direct assay
stopped assay
cycling assay
Orígen
Importado de HalCentros de investigación