Analysis of enzyme activities
Language
en
Chapitre d'ouvrage
This item was published in
Plant Metabolic Flux Analysis : Methods and Protocols, Plant Metabolic Flux Analysis : Methods and Protocols. 2014, vol. 1090
Springer Science+Business Media
English Abstract
The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the ...Read more >
The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented.Read less <
Keywords
microplate
English Keywords
enzyme activity
direct assay
stopped assay
cycling assay
Origin
Hal importedCollections