MENARD, Guillaume; BIAIS, Benoit; PRODHOMME, Duyên; BALLIAS, Patricia; GIBON, Yves

doi:10.1007/978-1-62703-688-7_15

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MENARD, Guillaume
Biologie du fruit et pathologie [BFP]

BIAIS, Benoit
Biologie du fruit et pathologie [BFP]

PRODHOMME, Duyên
Biologie du fruit et pathologie [BFP]

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Chapitre d'ouvrage

Ce document a été publié dans

Plant Metabolic Flux Analysis : Methods and Protocols, Plant Metabolic Flux Analysis : Methods and Protocols. 2014, vol. 1090

Springer Science+Business Media

Résumé en anglais

The evaluation of enzyme activities, especially their capacities, represents an important step towards the modelling of biochemical pathways in living organisms. The implementation of microplate technology enables the determination of up to >50 enzymes in relatively large numbers of samples and in various biological materials. Most of these enzymes are involved in central metabolism and several pathways are entirely covered. Direct or indirect assays can be used, as well as highly sensitive assays, depending on the abundance of the enzymes under study. To exemplify such methods, protocols for UDP-glucose pyrophosphorylase (E.C. 2.7.7.9) operating in real time and for pyrophosphate:fructose-6-phosphate 1-phosphotransferase (E.C. 2.7.1.90) are presented.< Réduire

Mots clés

microplate

Mots clés en anglais

enzyme activity

direct assay

stopped assay

cycling assay