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hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMALEMBIC-MAHER, Sylvie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCARLE, Patricia
dc.date.issued2013
dc.identifier.isbn978-1-62703-088-5 978-1-62703-089-2
dc.description.abstractEnPhysical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example.
dc.language.isoen
dc.publisherSpringer Science+Business Media
dc.publisher.locationNew York (united states)
dc.source.titlePhytoplasma: Methods and Protocols
dc.subjectflavescence dorée
dc.subjectmollicutes
dc.subject.enChromosome Mapping/ methods
dc.subject.enphytoplasma
dc.subject.engenetic map
dc.subject.enphysical map
dc.subject.enpulsed-field gel electrophoresis
dc.subject.enrestriction endonucleases
dc.subject.ensouthern blot
dc.title.enMapping the phytoplasma chromosome
dc.typeChapitre d'ouvrage
dc.identifier.doi10.1007/978-1-62703-089-2_34
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.volume938
bordeaux.title.proceedingPhytoplasma: Methods and Protocols
hal.identifierhal-02804649
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02804649v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Phytoplasma:%20Methods%20and%20Protocols&rft.date=2013&rft.volume=938&rft.au=MALEMBIC-MAHER,%20Sylvie&CARLE,%20Patricia&rft.isbn=978-1-62703-088-5%20978-1-62703-089-2&rft.genre=unknown


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