MALEMBIC-MAHER, Sylvie; CARLE, Patricia

doi:10.1007/978-1-62703-089-2_34

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

MALEMBIC-MAHER, Sylvie
Biologie du fruit et pathologie [BFP]

CARLE, Patricia
Biologie du fruit et pathologie [BFP]

Langue

Chapitre d'ouvrage

Ce document a été publié dans

Phytoplasma: Methods and Protocols, Phytoplasma: Methods and Protocols. 2013, vol. 938

Springer Science+Business Media

Résumé en anglais

Physical and genetic mapping of the phytoplasma chromosome can be a useful tool in a genome sequencing project in order to assemble the in silico-predicted contigs robustly. Mapping consists of four distinct steps: preparation of phytoplasma chromosomes from infected plants, single- and double-digestion of chromosomes with rare-cutting restriction enzymes, separation of large DNA fragments by pulsed-field gel electrophoresis, and hybridization with various genetic markers. Materials and methods needed for each step are described and the technique is illustrated using the flavescence doree phytoplasma genome map as an example.< Réduire

Mots clés

flavescence dorée

mollicutes

Mots clés en anglais

Chromosome Mapping/ methods

phytoplasma

genetic map

physical map

pulsed-field gel electrophoresis

restriction endonucleases

southern blot