BORNET, Aurélien; MAUCOURT, Mickael; DEBORDE, Catherine; JACOB, Daniel; MILANI, Jonas; VUICHAUD, Basile; JI, Xiao; DUMEZ, Jean-Nicolas; MOING, Annick; BODENHAUSEN, Geoffrey; JANNIN, Sami; GIRAUDEAU, Patrick

doi:10.1021/acs.analchem.6b01094

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BORNET, Aurélien
Institut des sciences et d'ingénierie chimiques [ISIC]

MAUCOURT, Mickael
Biologie du fruit et pathologie [BFP]
1074 Institut de Biologie Végétale Moléculaire : actions communes

DEBORDE, Catherine
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Analytical Chemistry. 2016-06, vol. 88, n° 12, p. 6179-6183

American Chemical Society

Résumé en anglais

At natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronudear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.< Réduire

DOI

http://dx.doi.org/10.1021/acs.analchem.6b01094

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332