Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics
MAUCOURT, Mickael
Biologie du fruit et pathologie [BFP]
1074 Institut de Biologie Végétale Moléculaire : actions communes
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Biologie du fruit et pathologie [BFP]
1074 Institut de Biologie Végétale Moléculaire : actions communes
MAUCOURT, Mickael
Biologie du fruit et pathologie [BFP]
1074 Institut de Biologie Végétale Moléculaire : actions communes
Biologie du fruit et pathologie [BFP]
1074 Institut de Biologie Végétale Moléculaire : actions communes
BODENHAUSEN, Geoffrey
Université Pierre et Marie Curie - Paris 6 [UPMC]
Département de Chimie - ENS Paris
< Reduce
Université Pierre et Marie Curie - Paris 6 [UPMC]
Département de Chimie - ENS Paris
Language
en
Article de revue
This item was published in
Analytical Chemistry. 2016-06, vol. 88, n° 12, p. 6179-6183
American Chemical Society
English Abstract
At natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization ...Read more >
At natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronudear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.Read less <
ANR Project
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
Origin
Hal importedCollections