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Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics

hal.structure.identifierInstitut des sciences et d'ingénierie chimiques [ISIC]
dc.contributor.authorBORNET, Aurélien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifier1074 Institut de Biologie Végétale Moléculaire : actions communes
dc.contributor.authorMAUCOURT, Mickael
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorJACOB, Daniel
hal.structure.identifierInstitut des sciences et d'ingénierie chimiques [ISIC]
dc.contributor.authorMILANI, Jonas
dc.contributor.authorVUICHAUD, Basile
hal.structure.identifierUniversité Pierre et Marie Curie - Paris 6 [UPMC]
dc.contributor.authorJI, Xiao
hal.structure.identifierInstitut de Chimie des Substances Naturelles [ICSN]
dc.contributor.authorDUMEZ, Jean-Nicolas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMOING, Annick
hal.structure.identifierUniversité Pierre et Marie Curie - Paris 6 [UPMC]
hal.structure.identifierDépartement de Chimie - ENS Paris
dc.contributor.authorBODENHAUSEN, Geoffrey
hal.structure.identifierInstitut des sciences et d'ingénierie chimiques [ISIC]
hal.structure.identifierBruker Biospin AG
dc.contributor.authorJANNIN, Sami
hal.structure.identifierChimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation [CEISAM]
dc.contributor.authorGIRAUDEAU, Patrick
dc.date.issued2016-06
dc.identifier.issn0003-2700
dc.description.abstractEnAt natural C-13 abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronudear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.title.enHighly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics
dc.typeArticle de revue
dc.identifier.doi10.1021/acs.analchem.6b01094
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
bordeaux.journalAnalytical Chemistry
bordeaux.page6179-6183
bordeaux.volume88
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-01366193
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01366193v1
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