NOTTELET, Pierre; BATAILLE, Laure; GOURGUES, Geraldine; ANGER, Robin; LARTIGUE, Carole; SIRAND-PUGNET, Pascal; MARZA, Esther; FRONZES, Remi; ARFI, Yonathan

doi:10.1126/sciadv.abf2403

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NOTTELET, Pierre
Institut Européen de Chimie et Biologie [IECB]

BATAILLE, Laure
Biologie du fruit et pathologie [BFP]

GOURGUES, Geraldine
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Science Advances. 2021, vol. 7, n° 10, p. eabf2403

American Association for the Advancement of Science (AAAS)

Résumé en anglais

Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V L and V H domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.< Réduire

Mots clés

Bactérie pathogène

Mots clés en anglais

Mycoplasme

Mollicutes

Mycoplasma immunoglobulin binding

MIB

mycoplasma immunoglobulin protease

MIP

DOI

http://dx.doi.org/10.1126/sciadv.abf2403

Project ANR

Dissection moléculaire du système de clivage d'anticorps des mycoplasmes - ANR-17-CE35-0002

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332