The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction
Language
en
Article de revue
This item was published in
Science Advances. 2021, vol. 7, n° 10, p. eabf2403
American Association for the Advancement of Science (AAAS)
English Abstract
Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H ...Read more >
Mycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V L and V H domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.Read less <
Keywords
Bactérie pathogène
English Keywords
Mycoplasme
Mollicutes
Mycoplasma immunoglobulin binding
MIB
mycoplasma immunoglobulin protease
MIP
ANR Project
Dissection moléculaire du système de clivage d'anticorps des mycoplasmes - ANR-17-CE35-0002
Origin
Hal importedCollections