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The mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction

hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
dc.contributor.authorNOTTELET, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATAILLE, Laure
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGOURGUES, Geraldine
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
dc.contributor.authorANGER, Robin
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIRAND-PUGNET, Pascal
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
dc.contributor.authorMARZA, Esther
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
dc.contributor.authorFRONZES, Remi
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARFI, Yonathan
dc.date.issued2021
dc.identifier.issn2375-2548
dc.description.abstractEnMycoplasma immunoglobulin binding (MIB) and mycoplasma immunoglobulin protease (MIP) are surface proteins found in the majority of mycoplasma species, acting sequentially to capture antibodies and cleave off their V H domains. Cryo-electron microscopy structures show how MIB and MIP bind to a Fab fragment in a "hug of death" mechanism. As a result, the orientation of the V L and V H domains is twisted out of alignment, disrupting the antigen binding site. We also show that MIB-MIP has the ability to promote the dissociation of the antibody-antigen complex. This system is functional in cells and protects mycoplasmas from antibody-mediated agglutination. These results highlight the key role of the MIB-MIP system in immunity evasion by mycoplasmas through an unprecedented mechanism, and open exciting perspectives to use these proteins as potential tools in the antibody field.
dc.description.sponsorshipDissection moléculaire du système de clivage d'anticorps des mycoplasmes - ANR-17-CE35-0002
dc.language.isoen
dc.publisherAmerican Association for the Advancement of Science (AAAS)
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectBactérie pathogène
dc.subject.enMycoplasme
dc.subject.enMollicutes
dc.subject.enMycoplasma immunoglobulin binding
dc.subject.enMIB
dc.subject.enmycoplasma immunoglobulin protease
dc.subject.enMIP
dc.title.enThe mycoplasma surface proteins MIB and MIP promote the dissociation of the antibody-antigen interaction
dc.typeArticle de revue
dc.identifier.doi10.1126/sciadv.abf2403
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie animale
dc.subject.halSciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animal
bordeaux.journalScience Advances
bordeaux.pageeabf2403
bordeaux.volume7
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-03162754
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03162754v1
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