Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode
BAKHROUF, Amina
Laboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir] [LATVPEP]
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Laboratoire d’analyse, de traitement et de valorisation des polluants de l’environnement et des produits [Monastir] [LATVPEP]
Language
en
Article de revue
This item was published in
Journal of Biosensors & Bioelectronics. 2015, vol. 6, n° 1, p. 1000160
Hilaris
English Abstract
In this work we describe a new urea biosensor, based on the immobilization of bacteria, Proteus mirabilis on gold electrode. To improve the stability of the bio-system, additional materials were used such as functionalized ...Read more >
In this work we describe a new urea biosensor, based on the immobilization of bacteria, Proteus mirabilis on gold electrode. To improve the stability of the bio-system, additional materials were used such as functionalized Fe 3 O 4 nanoparticles (NPs), cationic (PAH), anionic (PSS) polyelectrolytes, Bovine Serum Albumin (BSA) and glutaralde-hyde as a cross-linking agent. The electrochemical performances of the developed bacteria biosensor was evaluated using the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry measurements. The adhesion of the bacteria cell on gold electrode was evaluated using contact angle measurements. The morphology of bacteria and its interaction with Fe 3 O 4 nanoparticles were evaluated with the atomic force microscopy (AFM). As a result, a sensitive, stable and reproducible urea biosensor was developed.Read less <
English Keywords
Proteus mirabilis
Fe3O4
PAH and PSS polyelectrolytes
Impedance spectroscopy
Gold electrodes
Bacterial adhesion
Atomic Force Microscopy
Origin
Hal imported