Fluorescence microscopy of single autofluorescent proteins for cellular biology
Langue
en
Article de revue
Ce document a été publié dans
Comptes Rendus de l'Academie des Sciences. Série IV, Physique, Astronomie. 2002-08-15, vol. 3, p. 645
Elsevier
Résumé en anglais
In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed ...Lire la suite >
In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells.< Réduire
Mots clés en anglais
single-molecule
GFP
DsRed
fluorescence microscopy
two-photon excitation
fluorescence correlation spectroscopy.
fluorescence correlation spectroscopy
Origine
Importé de halUnités de recherche