COGNET, Laurent; COUSSEN, Françoise; CHOQUET, Daniel; LOUNIS, Brahim

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Licencia de uso del documento

COGNET, Laurent
Centre de physique moléculaire optique et hertzienne [CPMOH]

COUSSEN, Françoise
Physiologie cellulaire de la synapse [PCS]

CHOQUET, Daniel
Physiologie cellulaire de la synapse [PCS]

Idioma

Article de revue

Este ítem está publicado en

Comptes Rendus de l'Academie des Sciences. Série IV, Physique, Astronomie. 2002-08-15, vol. 3, p. 645

Elsevier

Resumen en inglés

In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells.< Leer menos

Palabras clave en inglés

single-molecule

GFP

DsRed

fluorescence microscopy

two-photon excitation

fluorescence correlation spectroscopy.

fluorescence correlation spectroscopy

Orígen

Importado de Hal

Centros de investigación

Laboratoire Ondes et Matière d'Aquitaine (LOMA) - UMR 5798