Fluorescence microscopy of single autofluorescent proteins for cellular biology
Language
en
Article de revue
This item was published in
Comptes Rendus de l'Academie des Sciences. Série IV, Physique, Astronomie. 2002-08-15, vol. 3, p. 645
Elsevier
English Abstract
In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed ...Read more >
In this paper we review the applicability of autofluorescent proteins for single-molecule imaging in biology. The photophysical characteristics of several mutants of the Green Fluorescent Protein (GFP) and those of DsRed are compared and critically discussed for their use in cellular biology. The alternative use of two-photon excitation at the single-molecule level or Fluorescence Correlation Spectroscopy is envisaged for the study of individual autofluorescent proteins. Single-molecule experiments performed in live cells using eGFP and preferably eYFP fusion proteins are reviewed. Finally, the first use at the single-molecule level of citrine, a more photostable variant of the eYFP is reported when fused to a receptor for neurotransmitter in live cells.Read less <
English Keywords
single-molecule
GFP
DsRed
fluorescence microscopy
two-photon excitation
fluorescence correlation spectroscopy.
fluorescence correlation spectroscopy
Origin
Hal imported