Resonant Waveguide Imaging of Living Systems: From Evanescent to Propagative Light
ARGOUL, Françoise
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
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Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
ARGOUL, Françoise
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
ARNEODO, Alain
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
< Reduce
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Language
en
Chapitre d'ouvrage
This item was published in
Handbook of Photonics for Biomedical Engineering, Handbook of Photonics for Biomedical Engineering. 2017-01p. 1-42
Springer Verlag
English Abstract
For more than 50 years, resonant waveguides (RWGs) have offered highly sensitive label-free sensing platforms to monitor surface processes such as protein adsorption, affinity binding, monolayer to multilayer build-up, ...Read more >
For more than 50 years, resonant waveguides (RWGs) have offered highly sensitive label-free sensing platforms to monitor surface processes such as protein adsorption, affinity binding, monolayer to multilayer build-up, bacteria and more generally adherent or confined living mammalian cells and tissues. Symmetrical planar dielectric RWG sensitivity was improved by metal coating of at least one of their surfaces for surface plasmon resonance undertaking (SPRWG). However, RWG sensitivity was often obtained at the expense of spatial resolution and could not compete with other high resolution fluorescence microscopies. For years, RWGs have only rarely been combined with high-resolution microscopy. Only recently, the improvement of intensity and phase light modulation techniques and the availability of low-cost high numerical aperture lenses have drastically changed the devices and methodologies based on RWGs. We illustrate in this chapter how these different technical and methodological evolutions have offered new, versatile, and powerful imaging tools to the biological community.Read less <
English Keywords
Evanescent field microscopy
Goss-Hanchen effet
Surface plasmon resonance
Guided-wave microscopy
High resolution imaging of living cells
Resonant waveguides
Origin
Hal imported