Tracking in real time the crawling dynamics of adherent living cells with a high resolution surface plasmon microscope
ARNEODO, A.
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
ARGOUL, Françoise
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
< Reduce
Laboratoire Ondes et Matière d'Aquitaine [LOMA]
Laboratoire de Physique de l'ENS Lyon [Phys-ENS]
Language
en
Communication dans un congrès
This item was published in
2016-04-22, San Francisco, CA. 2016-03-07, vol. 9724, p. 97240G (1-10)
Spie-Int Soc Optical Engineering
English Abstract
We introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer ...Read more >
We introduce a high resolution scanning surface plasmon microscope for long term imaging of living adherent mouse myoblast cells. The coupling of a high numerical aperture objective lens with a fibered heterodyne interferometer provides both enhanced sensitivity and long term stability. This microscope takes advantage of the plasmon resonance excitation and the amplification of the electromagnetic field in near-field distance to the gold coated coverslip. This plasmon enhanced evanescent wave microscopy is particularly attractive for the study of cell adhesion and motility since it can be operated without staining of the biological sample. We show that this microscope allows very long-term imaging of living samples, and that it can capture and follow the temporal deformation of C2C12 myoblast cell protusions (lamellipodia), during their migration on a at surface.Read less <
English Keywords
Dielectric polarisation
Surface plasmons
Microscopy
Polarisation
Interferometers
Dielectrics
Heterodyning
Origin
Hal imported