Photothermal Absorption Correlation Spectroscopy
Langue
en
Article de revue
Ce document a été publié dans
ACS Nano. 2009-02-24, vol. 3, n° 2, p. 345-350
American Chemical Society
Résumé en anglais
Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from ...Lire la suite >
Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from artifacts originating from the poor fluorophore photophysics: photobleaching, blinking, and saturation. To circumvent these limitations we present here a new correlation method called photothermal absorption correlation spectroscopy (PhACS) which relies on the absorption properties of tiny nano-objects. PhACS is based on the photothermal heterodyne detection technique and measures akin FCS, the time correlation function of the detected signals. Application of this technique to the precise determination of the hydrodynamic sizes of different functionalized gold nanoparticles are presented, highlighting the potential of this method.< Réduire
Mots clés en anglais
photothermal detection
correlation spectroscopy
single molecule
gold nanoparticles
Origine
Importé de halUnités de recherche