Looking for local adaptation: convergent microevolution in Aleppo pine (Pinus halepensis)
RUIZ DANIELS, Rose
Department of Forest Ecology and GeneticsForest Research Centre
The University of Edinburgh
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Department of Forest Ecology and GeneticsForest Research Centre
The University of Edinburgh
RUIZ DANIELS, Rose
Department of Forest Ecology and GeneticsForest Research Centre
The University of Edinburgh
< Reduce
Department of Forest Ecology and GeneticsForest Research Centre
The University of Edinburgh
Language
en
Article de revue
This item was published in
Genes. 2019, vol. 10, n° 9, p. 1-16
MDPI
English Abstract
Finding outlier loci underlying local adaptation is challenging and is best approached by suitable sampling design and rigorous method selection. In this study, we aimed to detect outlier loci (single nucleotide polymorphisms, ...Read more >
Finding outlier loci underlying local adaptation is challenging and is best approached by suitable sampling design and rigorous method selection. In this study, we aimed to detect outlier loci (single nucleotide polymorphisms, SNPs) at the local scale by using Aleppo pine (Pinus halepensis), a drought resistant conifer that has colonized many habitats in the Mediterranean Basin, as the model species. We used a nested sampling approach that considered replicated altitudinal gradients for three contrasting sites. We genotyped samples at 294 SNPs located in genomic regions selected to maximize outlier detection. We then applied three different statistical methodologies-Two Bayesian outlier methods and one latent factor principal component method-To identify outlier loci. No SNP was an outlier for all three methods, while eight SNPs were detected by at least two methods and 17 were detected only by one method. From the intersection of outlier SNPs, only one presented an allelic frequency pattern associated with the elevational gradient across the three sites. In a context of multiple populations under similar selective pressures, our results underline the need for careful examination of outliers detected in genomic scans before considering them as candidates for convergent adaptation.Read less <
English Keywords
Pinus halepensis
natural selection
outliers
SNP
altitudinal gradients
Origin
Hal imported