Primer note: A new set of highly polymorphic nuclear microsatellite markers for Nothofagus nervosa and related South American species
Language
en
Article de revue
This item was published in
Silvae Genetica. 2008, vol. 57, n° 2, p. 82-85
Walter De Gruyter
English Abstract
Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes ...Read more >
Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population. The primers transferred well to five related species (N. obliqua (Mirb.) Oerst, N. glauca (Phil.) Krasser, N. dombeyi (Mirb.) Oerst, N. pumilio (Poepp et Endl.) Krasser and N. antarctica (G. Forster) Oerst, with allele numbers up to 11. The high level of polymorphism promises a sufficient power for gene flow and parentage analyses.Read less <
Keywords
MICROSATELLITES
FINE-SCALE GENETIC STRUCTURE
AMPLIFICATION DE GÈNES
English Keywords
NOTHOFAGUS
SOUTH AMERICA
GENE FLOW
Origin
Hal imported