JOUSHOMME, Alexandre; GARENNE, André; DUFOSSÉE, Mélody; RENOM, Rémy; RUIGROK, Hermanus Johannes; CHAPPE, Yann Loick; CANOVI, Anne; PATRIGNONI, Lorenza; HURTIER, Annabelle; POULLETIER DE GANNES, Florence; LAGROYE, Isabelle; LÉVÊQUE, Philippe; LEWIS, Noëlle; PRIAULT, Muriel; ARNAUD-CORMOS, Delia; PERCHERANCIER, Yann

doi:10.3390/ijms23020658

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JOUSHOMME, Alexandre

Laboratoire de l'intégration, du matériau au système [IMS]

GARENNE, André
Laboratoire de l'intégration, du matériau au système [IMS]
Mnemonic Synergy [Mnemosyne]

DUFOSSÉE, Mélody
Institut de biochimie et génétique cellulaires [IBGC]

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International Journal of Molecular Medicine and Advanced Sciences. 2022-01, vol. 23, n° 2, p. 658

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It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-exposure to RF and chemical treatments known to induce either apoptosis or autophagy. Two human cell lines (SH-SY5Y and HCT116) and two cultures of primary rat cortex cells (astrocytes and co-culture of neurons and glial cells) were exposed to RF using an 1800 MHz carrier wave modulated with various environmental signals (GSM: Global System for Mobile Communications, 2G signal), UMTS (Universal Mobile Telecommunications System, 3G signal), LTE (Long-Term Evolution, 4G signal, and Wi-Fi) or unmodulated RF (continuous wave, CW). The specific absorption rates (S.A.R.) used were 1.5 and 6 W/kg during DHM experiments and ranged from 5 to 24 W/kg during the recording of cellular impedance. Cells were continuously exposed for three to five consecutive days while the temporal phenotypic signature of cells behavior was recorded at constant temperature. Statistical analysis of the results does not indicate that RF-EMF exposure impacted the global behavior of healthy, apoptotic, or autophagic cells, even at S.A.R. levels higher than the guidelines, provided that the temperature was kept constant.< Réduire

Mots clés en anglais

label-free techniques

radiofrequency electromagnetic fields

bioelectromagnetics

digital holographic microscopy

impedancemetry

human cell lines

rat cortex primary cells

apoptosis

autophagy

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https://oskar-bordeaux.fr/handle/20.500.12278/140465

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http://dx.doi.org/10.3390/ijms23020658

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Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

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Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

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