COUDON, Noemie; NAVAILLES, Laurence; NALLET, Frederic; LY, Isabelle; BENTALEB, Ahmed; CHAPEL, Jean-Paul; BEVEN, Laure; DOULIEZ, Jean-Paul; MARTIN, Nicolas

doi:10.1016/j.jcis.2022.02.138

Université de Bordeaux [UB]
Centre de Recherche Paul Pascal [CRPP]

NAVAILLES, Laurence

Centre de Recherche Paul Pascal [CRPP]

NALLET, Frederic

Centre de Recherche Paul Pascal [CRPP]

Langue

Article de revue

Ce document a été publié dans

Journal of Colloid and Interface Science. 2022, vol. 617, p. 257-266

Résumé en anglais

All-aqueous microdroplets produced by liquid–liquid phase separation have emerged as promising models of artificial cells, and offer new approaches for the solvent-free encapsulation of fragile solutes. Yet, the lack of a membrane on such droplets makes them intrinsically unstable against coarsening, and precludes a fine control over chemical localization, as solutes can freely diffuse through the interface. Herein, we report the construction of stable and impermeable water-in-water emulsions via the interfacial self-assembly of mixed sodium oleate/1-decanol bilayers on dextran-rich droplets produced by segregative liquid–liquid phase separation with poly(ethylene glycol). Lipids spontaneously self-assemble as multilamellar structures at the surface of the droplets as revealed by freeze-fracture transmission electron microscopy and small-angle X-ray scattering. We further demonstrate that the lipid-based membrane is impermeable to oligonucleotides and proteins, but also to a low molecular weight dye, so that a strict chemical encapsulation can be achieved by spontaneous partitioning within the droplets before membrane self-assembly. Taken together, our results highlight the ease of production of fatty acid-stabilized all-aqueous emulsions droplets able to encapsulate a range of solutes without the need of oil or organic solvents, paving the way to the construction of robust membrane-bounded, polymer-rich artificial cells.< Réduire

Mots clés en anglais

Liquid-liquid phase separation

Water-in-water emulsion

Fatty acids

Interfacial self-assembly

Artificial cells

URI

https://oskar-bordeaux.fr/handle/20.500.12278/140178

DOI

http://dx.doi.org/10.1016/j.jcis.2022.02.138

Project ANR

Compartiments Hybrides comme Cellules Synthétiques - ANR-19-CE06-0013
Initiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003

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Stabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers

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Stabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers

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Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Project ANR

Unités de recherche