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Stabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers

dc.rights.licenseopenen_US
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorCOUDON, Noemie
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorNAVAILLES, Laurence
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorNALLET, Frederic
IDREF: 068545487
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorLY, Isabelle
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBENTALEB, Ahmed
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorCHAPEL, Jean-Paul
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDOULIEZ, Jean-Paul
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorMARTIN, Nicolas
dc.date.accessioned2022-06-10T07:00:58Z
dc.date.available2022-06-10T07:00:58Z
dc.date.issued2022
dc.identifier.issn219797en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/140178
dc.description.abstractEnAll-aqueous microdroplets produced by liquid–liquid phase separation have emerged as promising models of artificial cells, and offer new approaches for the solvent-free encapsulation of fragile solutes. Yet, the lack of a membrane on such droplets makes them intrinsically unstable against coarsening, and precludes a fine control over chemical localization, as solutes can freely diffuse through the interface. Herein, we report the construction of stable and impermeable water-in-water emulsions via the interfacial self-assembly of mixed sodium oleate/1-decanol bilayers on dextran-rich droplets produced by segregative liquid–liquid phase separation with poly(ethylene glycol). Lipids spontaneously self-assemble as multilamellar structures at the surface of the droplets as revealed by freeze-fracture transmission electron microscopy and small-angle X-ray scattering. We further demonstrate that the lipid-based membrane is impermeable to oligonucleotides and proteins, but also to a low molecular weight dye, so that a strict chemical encapsulation can be achieved by spontaneous partitioning within the droplets before membrane self-assembly. Taken together, our results highlight the ease of production of fatty acid-stabilized all-aqueous emulsions droplets able to encapsulate a range of solutes without the need of oil or organic solvents, paving the way to the construction of robust membrane-bounded, polymer-rich artificial cells.
dc.description.sponsorshipCompartiments Hybrides comme Cellules Synthétiques - ANR-19-CE06-0013en_US
dc.description.sponsorshipInitiative d'excellence de l'Université de Bordeauxen_US
dc.language.isoENen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.subject.enLiquid-liquid phase separation
dc.subject.enWater-in-water emulsion
dc.subject.enFatty acids
dc.subject.enInterfacial self-assembly
dc.subject.enArtificial cells
dc.title.enStabilization of all-aqueous droplets by interfacial self-assembly of fatty acids bilayers
dc.title.alternativeJ. Colloid Interface Sci.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.jcis.2022.02.138en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalJournal of Colloid and Interface Scienceen_US
bordeaux.page257-266en_US
bordeaux.volume617en_US
bordeaux.hal.laboratoriesCentre de Recherche Paul Pascal (CRPP) - UMR 5031en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINRAE
bordeaux.teamColloïdes, interfaces, assemblages (CIA)
bordeaux.teamMatière molle: structure et dynamique (M2SD)
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03626236
hal.version1
hal.exporttrue
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