THEODORAKOPOULOS, Nicolas; CHAPON, Virginie; COPPIN, Frederic; FLORIANI, Magali; VERCOUTER, Thomas; SERGEANT, Claire; CAMILLERI, Virginie; BERTHOMIEU, C.; FEVRIER, Laureline

doi:10.1016/j.jhazmat.2014.12.018

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THEODORAKOPOULOS, Nicolas
Interactions Protéine Métal [IPM]
Biologie végétale et microbiologie environnementale - UMR7265 [BVME]
Laboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]

CHAPON, Virginie
Interactions Protéine Métal [IPM]
Biologie végétale et microbiologie environnementale - UMR7265 [BVME]

COPPIN, Frederic
Laboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]

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Ce document a été publié dans

Journal of Hazardous Materials. 2015-03-21, vol. 285, p. 285-293

Elsevier

Résumé en anglais

Although uranium (U) is naturally found in the environment, soil remediation programs will become increasingly important in light of certain human activities. This work aimed to identify U(VI) detoxification mechanisms employed by a bacteria strain isolated from a Chernobyl soil sample, and to distinguish its active from passive mechanisms of interaction. The ability of the Microbacterium sp. A9 strain to remove U(VI) from aqueous solutions at 4 °C and 25 °C was evaluated, as well as its survival capacity upon U(VI) exposure. The subcellular localisation of U was determined by TEM/EDX microscopy, while functional groups involved in the interaction with U were further evaluated by FTIR; finally, the speciation of U was analysed by TRLFS. We have revealed, for the first time, an active mechanism promoting metal efflux from the cells, during the early steps following U(VI) exposure at 25 °C. The Microbacterium sp. A9 strain also stores U intracellularly, as needle-like structures that have been identified as an autunite group mineral. Taken together, our results demonstrate that this strain exhibits a high U(VI) tolerance based on multiple detoxification mechanisms. These findings support the potential role of the genus Microbacterium in the remediation of aqueous environments contaminated with U(VI) under aerobic conditions.< Réduire

Mots clés en anglais

uranium

microbacterium

detoxication mechanisms

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Use of combined microscopic and spectroscopic techniques to reveal interactions between uranium and Microbacterium sp. A9, a strain isolated from the Chernobyl exclusion zone

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