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Use of combined microscopic and spectroscopic techniques to reveal interactions between uranium and Microbacterium sp. A9, a strain isolated from the Chernobyl exclusion zone

hal.structure.identifierInteractions Protéine Métal [IPM]
hal.structure.identifierBiologie végétale et microbiologie environnementale - UMR7265 [BVME]
hal.structure.identifierLaboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]
dc.contributor.authorTHEODORAKOPOULOS, Nicolas
hal.structure.identifierInteractions Protéine Métal [IPM]
hal.structure.identifierBiologie végétale et microbiologie environnementale - UMR7265 [BVME]
dc.contributor.authorCHAPON, Virginie
hal.structure.identifierLaboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]
dc.contributor.authorCOPPIN, Frederic
hal.structure.identifierLaboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]
dc.contributor.authorFLORIANI, Magali
hal.structure.identifierLaboratoire de développement Analytique Nucléaire Isotopique et Elémentaire [LANIE]
dc.contributor.authorVERCOUTER, Thomas
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorSERGEANT, Claire
hal.structure.identifierLaboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]
dc.contributor.authorCAMILLERI, Virginie
hal.structure.identifierInteractions Protéine Métal [IPM]
hal.structure.identifierBiologie végétale et microbiologie environnementale - UMR7265 [BVME]
dc.contributor.authorBERTHOMIEU, C.
hal.structure.identifierLaboratoire de Biogéochimie, Biodisponibilité et Transfert des Radionucléides [IRSN/PRP-ENV/SERIS/L2BT]
dc.contributor.authorFEVRIER, Laureline
dc.date.issued2015-03-21
dc.identifier.issn0304-3894
dc.description.abstractEnAlthough uranium (U) is naturally found in the environment, soil remediation programs will become increasingly important in light of certain human activities. This work aimed to identify U(VI) detoxification mechanisms employed by a bacteria strain isolated from a Chernobyl soil sample, and to distinguish its active from passive mechanisms of interaction. The ability of the Microbacterium sp. A9 strain to remove U(VI) from aqueous solutions at 4 °C and 25 °C was evaluated, as well as its survival capacity upon U(VI) exposure. The subcellular localisation of U was determined by TEM/EDX microscopy, while functional groups involved in the interaction with U were further evaluated by FTIR; finally, the speciation of U was analysed by TRLFS. We have revealed, for the first time, an active mechanism promoting metal efflux from the cells, during the early steps following U(VI) exposure at 25 °C. The Microbacterium sp. A9 strain also stores U intracellularly, as needle-like structures that have been identified as an autunite group mineral. Taken together, our results demonstrate that this strain exhibits a high U(VI) tolerance based on multiple detoxification mechanisms. These findings support the potential role of the genus Microbacterium in the remediation of aqueous environments contaminated with U(VI) under aerobic conditions.
dc.language.isoen
dc.publisherElsevier
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enuranium
dc.subject.enmicrobacterium
dc.subject.endetoxication mechanisms
dc.title.enUse of combined microscopic and spectroscopic techniques to reveal interactions between uranium and Microbacterium sp. A9, a strain isolated from the Chernobyl exclusion zone
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jhazmat.2014.12.018
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalJournal of Hazardous Materials
bordeaux.page285-293
bordeaux.volume285
bordeaux.peerReviewedoui
hal.identifierhal-01561096
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01561096v1
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