FOUILLEN, Laetitia; CLAVEROL, Stéphane; BAYER, Emmanuelle M F; GRISON, Magali

doi:10.1007/978-1-0716-2132-5_12

Laboratoire de biogenèse membranaire [LBM]

CLAVEROL, Stéphane

BAYER, Emmanuelle M F

Laboratoire de biogenèse membranaire [LBM]

Langue

Chapitre d'ouvrage

Ce document a été publié dans

Plasmodesmata. Methods in Molecular Biology. 2022-03, vol. 2457, p. 189-207

Springer

Résumé en anglais

Plasmodesmata (PD) are membranous intercellular nanochannels crossing the plant cell wall to connect adjacent cells in plants. Our understanding of PD function heavily relies on the identification of their molecular components, these being proteins or lipids. In that regard, proteomic and lipidomic analyses of purified PD represent a crucial strategy in the field. Here we describe a simple two-step purification procedure that allows isolation of pure PD-derived membranes from Arabidopsis suspension cells suitable for "omic" approaches. The first step of this procedure consists on isolating pure cell walls containing intact PD, followed by a second step which involves an enzymatic degradation of the wall matrix to release PD membranes. The PD-enriched fraction can then serve to identify the lipid and protein composition of PD using lipidomic and proteomic approaches, which we also describe in this method article.< Réduire

Mots clés en anglais

Plasmodesmata

Membrane

Cell wall

Cellulase

Suspension cell

Proteomic

Lipidomic

Mass spectrometry

Arabidopsis thaliana

URI

https://oskar-bordeaux.fr/handle/20.500.12278/136590

DOI

http://dx.doi.org/10.1007/978-1-0716-2132-5_12

Project ANR

CONTACTS MEMBRANAIRES ET LE CONTROLE DE LA COMMUNICATION INTERCELLULAIRE CHEZ LES PLANTES - ANR-18-CE13-0016
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

Unités de recherche

Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200

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Isolation of Plasmodesmata membranes for lipidomic and proteomic analysis