Isolation of Plasmodesmata membranes for lipidomic and proteomic analysis
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | FOUILLEN, Laetitia
ORCID: 0000-0002-1204-9296 IDREF: 136936385 | |
| dc.contributor.author | CLAVEROL, Stéphane | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BAYER, Emmanuelle M F | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | GRISON, Magali | |
| dc.date.accessioned | 2022-03-31T16:23:29Z | |
| dc.date.available | 2022-03-31T16:23:29Z | |
| dc.date.issued | 2022-03 | |
| dc.identifier.isbn | 978-1-0716-2132-5 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/136590 | |
| dc.description.abstractEn | Plasmodesmata (PD) are membranous intercellular nanochannels crossing the plant cell wall to connect adjacent cells in plants. Our understanding of PD function heavily relies on the identification of their molecular components, these being proteins or lipids. In that regard, proteomic and lipidomic analyses of purified PD represent a crucial strategy in the field. Here we describe a simple two-step purification procedure that allows isolation of pure PD-derived membranes from Arabidopsis suspension cells suitable for "omic" approaches. The first step of this procedure consists on isolating pure cell walls containing intact PD, followed by a second step which involves an enzymatic degradation of the wall matrix to release PD membranes. The PD-enriched fraction can then serve to identify the lipid and protein composition of PD using lipidomic and proteomic approaches, which we also describe in this method article. | |
| dc.description.sponsorship | CONTACTS MEMBRANAIRES ET LE CONTROLE DE LA COMMUNICATION INTERCELLULAIRE CHEZ LES PLANTES - ANR-18-CE13-0016 | en_US |
| dc.description.sponsorship | Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010 | en_US |
| dc.language.iso | EN | en_US |
| dc.publisher | Springer | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.source.title | Plasmodesmata. Methods in Molecular Biology | en_US |
| dc.subject.en | Plasmodesmata | |
| dc.subject.en | Membrane | |
| dc.subject.en | Cell wall | |
| dc.subject.en | Cellulase | |
| dc.subject.en | Suspension cell | |
| dc.subject.en | Proteomic | |
| dc.subject.en | Lipidomic | |
| dc.subject.en | Mass spectrometry | |
| dc.subject.en | Arabidopsis thaliana | |
| dc.title.en | Isolation of Plasmodesmata membranes for lipidomic and proteomic analysis | |
| dc.title.alternative | Methods Mol Biol | en_US |
| dc.type | Chapitre d'ouvrage | en_US |
| dc.identifier.doi | 10.1007/978-1-0716-2132-5_12 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie cellulaire | en_US |
| dc.identifier.pubmed | 35349141 | en_US |
| bordeaux.page | 189-207 | en_US |
| bordeaux.volume | 2457 | en_US |
| bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-03626801 | |
| hal.version | 1 | |
| hal.date.transferred | 2022-04-01T07:54:44Z | |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | CC BY-NC-ND | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Plasmodesmata.%20Methods%20in%20Molecular%20Biology&rft.date=2022-03&rft.volume=2457&rft.spage=189-207&rft.epage=189-207&rft.au=FOUILLEN,%20Laetitia&CLAVEROL,%20St%C3%A9phane&BAYER,%20Emmanuelle%20M%20F&GRISON,%20Magali&rft.isbn=978-1-0716-2132-5&rft.genre=unknown |
