Show simple item record

dc.rights.licenseopenen_US
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorSAMOT, Johan
dc.contributor.authorROUABHIA, Mahmoud
dc.date.accessioned2022-03-02T14:49:57Z
dc.date.available2022-03-02T14:49:57Z
dc.date.issued2021-02
dc.identifier.issn1867-1314en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/128797
dc.description.abstractEnIncreasing resistance and changes in the spectrum of Candida infections have generated considerable interest in the development of new antifungal molecules. The use of antimicrobial peptides (AMPs) appears to be a promising approach. Frog skin AMPs (such as dermaseptins) have shown antimicrobial activity against several pathogens. In this study, we aimed to test the antimicrobial efficacy of dermaseptin S4 (DS4) against C. albicans. We determined the minimal inhibitory concentration (MIC) of DS4, and investigated the effects of the DS4 at low concentrations on human primary gingival fibroblasts. Additionally, we evaluated the effect of DS4 on C. albicans growth, form changes, and biofilm formation, as well as the expression of certain virulent genes. Our data show that DS4 completely inhibits C. albicans growth at a concentration of 32 µg/mL referring to the MIC of DS4. It should be noted that even with low concentrations (below 16 µg/mL), DS4 still have significant growth reduction of C. albicans, but were not toxic to human gingival fibroblasts. DS4 inhibited the transition from yeast to hyphae, and decreased the biofilm formation by reducing the biofilm mass weight. Surface morphological changes in the yeast cell membrane were observed following exposure to DS4. The gene expression analyses revealed that DS4 significantly decreased the expression of EAP1 and HWP1 genes. Overall results suggest the potential use of DS4 as an antifungal therapy to prevent C. albicans pathogenesis.
dc.language.isoENen_US
dc.subject.enAntimicrobial cationic peptides
dc.subject.enCandida albicans
dc.subject.enBiofilm
dc.subject.enGingival fibroblast
dc.subject.enEAP1
dc.subject.enHWP1
dc.subject.enGenes
dc.title.enEffect of Dermaseptin S4 on C. albicans Growth and EAP1 and HWP1 Gene Expression
dc.title.alternativeProbiotics & Antimicro. Prot.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s12602-020-09685-0en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalProbiotics and Antimicrobial Proteinsen_US
bordeaux.page287-298en_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesUnité de Recherche Oenologie - EA 4577en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Probiotics%20and%20Antimicrobial%20Proteins&rft.date=2021-02&rft.volume=13&rft.issue=1&rft.spage=287-298&rft.epage=287-298&rft.eissn=1867-1314&rft.issn=1867-1314&rft.au=SAMOT,%20Johan&ROUABHIA,%20Mahmoud&rft.genre=article


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record