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Evaluation of three Brettanomyces qPCR commercial kits: results from an interlaboratory study

dc.rights.licenseopenen_US
dc.contributor.authorLONGIN, Cedric
dc.contributor.authorJULLIAT, Frederique
dc.contributor.authorSERPAGGI, Virginie
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMAUPEU, Julie
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorBOURBON, Geoffrey
dc.contributor.authorROUSSEAUX, Sandrine
dc.contributor.authorGUILLOUX-BENATIER, Michele
dc.contributor.authorALEXANDRE, Herve
dc.date.accessioned2021-09-17T12:27:19Z
dc.date.available2021-09-17T12:27:19Z
dc.date.issued2016-12-21
dc.identifier.issn2494-1271en_US
dc.identifier.urioai:crossref.org:10.20870/oeno-one.2016.50.4.1274
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/112234
dc.description.abstractEnAim: Brettanomyces bruxellensis is well adapted to high ethanol concentrations and low pH which allows it to develop in difficult environments, such as wine. B. bruxellensis is mainly found in red wine and is regarded as a spoilage yeast due to its production of ethylphenols and other compounds responsible for organoleptic defects. The detection and quantification of this yeast is essential to preventing wine spoilage. Several specific detection and quantification kits based on real time quantitative PCR (qPCR) are commercially available. Although these kits are frequently used by private enological and research laboratories, no scientific reports on the reliability and performance of these kits, including interlaboratory and interassay comparisons, have been published. The aim of this work was to compare commercially available kits for the quantification of B. bruxellensis in red wine to classical method (plate counting on selective medium) in an interlaboratory study. Methods and results: Three different commercial kits were tested on three different wines from Bordeaux, Cotes du Rhone, and Burgundy inoculated with B. bruxellensis at four different concentrations. Five naturally contaminated wines from different French wine regions were also tested. Our results suggest that all the kits tested probably over or underestimate the quantity of B. bruxellensis in red wine and, under specific conditions, give false positives. Conclusion: Quantification may be very heterogeneous depending on the wine, laboratory, or population level. Underestimations or false negative results may have serious consequences for winemakers. Overestimation may be partly due to the quantification of dead cells by qPCR. Significance and impact of the study: This study highlights that quantification of B. bruxellensis in red wine using commercial kits requires a high level of expertise in molecular biology. We recommend that all users use a microbiological internal control to validate DNA extraction yield.
dc.language.isoENen_US
dc.sourcecrossref
dc.subjectBrettanomyces Bruxellensis
dc.subjectCommercial Kits
dc.subjectDNA Extraction
dc.subjectQuantitative PCR
dc.subjectRed Wine
dc.title.enEvaluation of three Brettanomyces qPCR commercial kits: results from an interlaboratory study
dc.typeArticle de revueen_US
dc.identifier.doi10.20870/oeno-one.2016.50.4.1274en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalOENO Oneen_US
bordeaux.volume50en_US
bordeaux.hal.laboratoriesUnité de Recherche Oenologie - EA 4577en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
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