JARRIAULT, David; FULLER, Justine; HYLAND, Brian I; MERCER, Alison R

doi:10.1038/s41598-018-34460-1

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JARRIAULT, David
Nutrition et Neurobiologie intégrée [NutriNeuro]

FULLER, Justine

HYLAND, Brian I

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Article de revue

Ce document a été publié dans

Scientific Reports. 2018-11-02, vol. 8, n° 1, p. 16277

Résumé en anglais

In Drosophila melanogaster, aversive (electric shock) stimuli have been shown to activate subpopulations of dopaminergic neurons with terminals in the mushroom bodies (MBs) of the brain. While there is compelling evidence that dopamine (DA)-induced synaptic plasticity underpins the formation of aversive memories in insects, the mechanisms involved have yet to be fully resolved. Here we take advantage of the accessibility of MBs in the brain of the honey bee to examine, using fast scan cyclic voltammetry, the kinetics of DA release and reuptake in vivo in response to electric shock, and to investigate factors that modulate the release of this amine. DA increased transiently in the MBs in response to electric shock stimuli. The magnitude of release varied depending on stimulus duration and intensity, and a strong correlation was identified between DA release and the intensity of behavioural responses to shock. With repeated stimulation, peak DA levels increased. However, the amount of DA released on the first stimulation pulse typically exceeded that evoked by subsequent pulses. No signal was detected in response to odour alone. Interestingly, however, if odour presentation was paired with electric shock, DA release was enhanced. These results set the stage for analysing the mechanisms that modulate DA release in the MBs of the bee.< Réduire

Mots clés en anglais

Animals

Bees

Behavior

Animal

Conditioning

Psychological

Dopamine

Dopamine Uptake Inhibitors

Dopaminergic Neurons

Electrodes

Electroshock

Male

Mushroom Bodies

Neuronal Plasticity

Nomifensine

Odorants