Resolvin D1 and E1 promote resolution of inflammation in microglial cells in vitro
Langue
EN
Article de revue
Ce document a été publié dans
Brain, Behavior, and Immunity. 2016, vol. 55, p. 249-259
Résumé en anglais
Sustained inflammation in the brain together with microglia activation can lead to neuronal damage. Hence limiting brain inflammation and activation of microglia is a real therapeutic strategy for inflammatory disease. ...Lire la suite >
Sustained inflammation in the brain together with microglia activation can lead to neuronal damage. Hence limiting brain inflammation and activation of microglia is a real therapeutic strategy for inflammatory disease. Resolvin D1 (RvD1) and resolvin E1 (RvE1) derived from n-3 long chain polyunsaturated fatty acids are promising therapeutic compounds since they actively turn off the systemic inflammatory response. We thus evaluated the anti-inflammatory activities of RvD1 and RvE1 in microglia cells in vitro. BV2 cells were pre-incubated with RvD1 or RvE1 before lipopolysaccharide (LPS) treatment. RvD1 and RvE1 both decreased LPS-induced proinflammatory cytokines (TNF-α, IL-6 and IL-1β) gene expression, suggesting their proresolutive activity in microglia. However, the mechanisms involved are distinct as RvE1 regulates NFκB signaling pathway and RvD1 regulates miRNAs expression. Overall, our findings support that pro-resolving lipids are involved in the resolution of brain inflammation and can be considered as promising therapeutic agents for brain inflammation.< Réduire
Mots clés en anglais
Animals
BV2
DHA
Docosahexaenoic Acids
Eicosapentaenoic Acid
EPA
Inflammation
Interleukin-6
Mice
Microglia
Microglial cells
MicroRNAs
miRNA
n-3 PUFA
Neuroinflammation
RvD1
RvE1
Tumor Necrosis Factor-alpha
Unités de recherche