ZHU, Ying; MESZAROS, Jozsef; WALLE, Roman; FAN, Rongxi; SUN, Ziyi; DWORK, Andrew J.; TRIFILIEFF, Pierre; JAVITCH, Jonathan A.

doi:10.2144/btn-2019-0083

Nutrition et Neurobiologie intégrée [NutriNeuro]

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Article de revue

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Biotechniques. 2019-12-20, vol. 68, n° 3, p. 122-129

Résumé en anglais

Despite the controversy regarding the existence and physiological relevance of class A G protein-coupled receptor dimerization, there is substantial evidence for functional interactions between the dopamine D2 receptor (D2R) and the adenosine A2A receptor (A2AR). A2AR-D2R complexes have been detected in rodent brains by proximity ligation assay; however, their existence in the human brain has not been demonstrated. In this study, we used Brightfield proximity ligation assay, combined with a systematic sampling and a parameter-free naive Bayesian classifier, and demonstrated proximity between the D2R and the A2AR in the adult human ventral striatum, consistent with their colocalization within complexes and the possible existence of D2R-A2AR heteromers. These methods are applicable to the relative quantification of proximity of two proteins, as well as the expression levels of individual proteins.< Réduire

Mots clés en anglais

Adenosine

Adenosine A2A receptor

Dopamine

Dopamine receptor type 2

G protein-coupled receptor

Machine learning

Naive Bayesian classifier

Proximity ligation assay

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https://oskar-bordeaux.fr/handle/20.500.12278/110267

DOI

http://dx.doi.org/10.2144/btn-2019-0083

Unités de recherche

NutriNeuro (Laboratoire de Nutrition et Neurobiologie Intégrée) - UMR 1286

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Detecting G protein-coupled receptor complexes in postmortem human brain with proximity ligation assay and a Bayesian classifier

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Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche