PULKA-ZIACH, Karolina; PAVET, Valeria; CHEKKAT, Neila; ESTIEU-GIONNET, Karine; ROHAC, Roman; LECHNER, Marie-Charlotte; SMULSKI, Cristian R.; ZEDER-LUTZ, Gabrielle; ALTSCHUH, Daniele; GRONEMEYER, Hinrich; FOURNEL, Sylvie; ODAERT, Benoit; GUICHARD, Gilles

doi:10.1002/cbic.201402485

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

PULKA-ZIACH, Karolina

PAVET, Valeria

CHEKKAT, Neila

Langue

Article de revue

Ce document a été publié dans

Chembiochem. 2015, vol. 16, n° 2, p. 293-301

Résumé en anglais

Cyclic peptides containing redox-stable thioether bridges might provide a useful alternative to disulfide-bridged bioactive peptides. We report the effect of replacing the disulfide bridge with a lanthionine linkage in a 16-mer cyclic peptide that binds to death receptor 5 (DR5, TRAIL-R2). Upon covalent oligomerisation, the disulfide-bridged peptide has previously shown similar behaviour to that of TNF-related apoptosis inducing ligand (TRAIL), by selectively triggering the DR5 cell death pathway. The structural and biological properties of the DR5-binding peptide and its desulfurised analogue were compared. Surface plasmon resonance (SPR) data suggest that these peptides bind DR5 with comparable affinities. The same holds true for dimeric versions of these peptides: the thioether is able to induce DR5-mediated apoptosis of BJAB lymphoma and tumorigenic BJELR cells, albeit to a slightly lower extent compared to its disulfide homologue. NMR analysis revealed subtle variation in the conformations of the two peptides and suggests that the thioether peptide is slightly less folded than its disulfide homologue. These observations could account for the different capability of the two dimers to cluster DR5 receptors on the cell surface and to trigger apoptosis. Nevertheless, our results suggest that the thioether peptide is a potential candidate for evaluation in animal models.< Réduire

Métadonnées

Partager cette publication !

Licence d’utilisation du document

Thioether Analogues of Disulfide-Bridged Cyclic Peptides Targeting Death Receptor 5: Conformational Analysis, Dimerisation and Consequences for Receptor Activation

Langue

Ce document a été publié dans

Résumé en anglais

URI

DOI

Unités de recherche