LIU, Liwang; PLAWINSKI, Laurent; DURRIEU, Marie-Christine; AUDOIN, Bertrand

doi:10.1002/jbio.201900045

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LIU, Liwang

PLAWINSKI, Laurent

DURRIEU, Marie-Christine
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

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Journal of Biophotonics. 2019, vol. 12, n° 8, p. e201900045-e201900045

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Advances in microscopy with new visualization possibilities often bring dramatic progress to our understanding of the intriguing cellular machinery. Picosecond optoacoustic micro-spectroscopy is an optical technique based on ultrafast pump-probe generation and detection of hypersound on time durations of picoseconds and length scales of nanometers. It is experiencing a renaissance as a versatile imaging tool for cell biology research after a plethora of applications in solid-state physics. In this emerging context, this work reports on a dual-probe architecture to carry out real-time parallel detection of the hypersound propagation inside a cell that is cultured on a metallic substrate, and of the hypersound reflection at the metal/cell adhesion interface. Using this optoacoustic modality, several biophysical properties of the cell can be measured in a noncontact and label-free manner. Its abilities are demonstrated with the multiple imaging of a mitotic macrophage-like cell in a single run experiment.< Réduire

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cell adhesion

cell mechanics

mitosis and division

picosecond optoacoustics

single-cell imaging

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Label-free multi-parametric imaging of single cells: dual picosecond optoacoustic microscopy

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