Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | CACAS, Jean-Luc | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | BURE, Corinne | |
dc.contributor.author | GROSJEAN, Kevin | |
dc.contributor.author | GERBEAU-PISSOT, Patricia | |
dc.contributor.author | LHERMINIER, Jeannine | |
dc.contributor.author | ROMBOUTS, Yoann | |
dc.contributor.author | MAES, Emmanuel | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | BOSSARD, Claire | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | GRONNIER, Julien | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | FURT, Fabienne | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | FOUILLEN, Laetitia | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | GERMAIN, Veronique | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | BAYER, Emmanuelle | |
hal.structure.identifier | Unité de Recherche Oenologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | CLUZET, Stephanie | |
dc.contributor.author | ROBERT, Franck | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | SCHMITTER, Jean-Marie | |
dc.contributor.author | DELEU, Magali | |
dc.contributor.author | LINS, Laurence | |
dc.contributor.author | SIMON-PLAS, Francoise | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | MONGRAND, Sébastien | |
dc.date.accessioned | 2020-09-03T07:56:26Z | |
dc.date.available | 2020-09-03T07:56:26Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 1532-2548 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/10899 | |
dc.description.abstractEn | The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 ± 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed. 2016 American Society of Plant Biologists. All Rights Reserved. | |
dc.language.iso | en | |
dc.title.en | Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1104/pp.15.00564 | |
dc.subject.hal | Chimie/Matériaux | |
bordeaux.journal | Plant physiology | |
bordeaux.page | 367-84 | |
bordeaux.volume | 170 | |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | * |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN, UMR 5248) | |
bordeaux.issue | 1 | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | INRAE | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20physiology&rft.date=2016&rft.volume=170&rft.issue=1&rft.spage=367-84&rft.epage=367-84&rft.eissn=1532-2548&rft.issn=1532-2548&rft.au=CACAS,%20Jean-Luc&BURE,%20Corinne&GROSJEAN,%20Kevin&GERBEAU-PISSOT,%20Patricia&LHERMINIER,%20Jeannine&rft.genre=article |
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