CACAS, Jean-Luc; BURE, Corinne; GROSJEAN, Kevin; GERBEAU-PISSOT, Patricia; LHERMINIER, Jeannine; ROMBOUTS, Yoann; MAES, Emmanuel; BOSSARD, Claire; GRONNIER, Julien; FURT, Fabienne; FOUILLEN, Laetitia; GERMAIN, Veronique; BAYER, Emmanuelle; CLUZET, Stephanie; ROBERT, Franck; SCHMITTER, Jean-Marie; DELEU, Magali; LINS, Laurence; SIMON-PLAS, Francoise; MONGRAND, Sébastien

doi:10.1104/pp.15.00564

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CACAS, Jean-Luc
Laboratoire de biogenèse membranaire [LBM]

BURE, Corinne
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

GROSJEAN, Kevin

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Plant physiology. 2016, vol. 170, n° 1, p. 367-84

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The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 ± 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed. 2016 American Society of Plant Biologists. All Rights Reserved.< Leer menos

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https://oskar-bordeaux.fr/handle/20.500.12278/10899

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http://dx.doi.org/10.1104/pp.15.00564

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Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

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Revisiting Plant Plasma Membrane Lipids in Tobacco: A Focus on Sphingolipids

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Centros de investigación