WADEESIRISAK, Kanthida; CASTANO, Sabine; BERTHELOT, Karine; VAYSSE, Laurent; BONFILS, Frederic; PERUCH, Frederic; RATTANAPORN, Kittipong; LIENGPRAYOON, Siriluck; LECOMTE, Sophie; BOTTIER, Celine

doi:10.1016/j.bbamem.2016.11.010

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WADEESIRISAK, Kanthida

CASTANO, Sabine

Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

BERTHELOT, Karine
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

Langue

Article de revue

Ce document a été publié dans

Biochimica Et Biophysica Acta-Biomembranes. 2017-02

Résumé en anglais

Rubber particle membranes from the Hevea latex contain predominantly two proteins, REF1 and SRPP1 involved in poly(cis-1,4-isoprene) synthesis or rubber quality. The repartition of both proteins on the small or large rubber particles seems to differ, but their role in the irreversible coagulation of the rubber particle is still unknown. In this study we highlighted the different modes of interactions of both recombinant proteins with different classes of lipids extracted from Hevea brasiliensis latex, and defined as phospholipids (PL), glycolipids (GL) and neutral lipids (NL). We combined two biophysical methods, polarization modulated-infrared reflection adsorption spectroscopy (PM-IRRAS) and ellipsometry to elucidate their interactions with monolayers of each class of lipids. REF1 and SRPP1 interactions with native lipids are clearly different; SRPP1 interacts mostly in surface with PI, GL or NL, without modification of its structure. In contrast REF1 inserts deeply in the lipid monolayers with all lipid classes. With NI, REF1 is even able to switch from alpha-helice conformation to beta-sheet structure, as in its aggregated form (amyloid form). Interaction between REF1 and NL may therefore have a specific role in the irreversible coagulation of rubber particles.< Réduire

Mots clés en anglais

Hevea brasiliensis rubber particle proteins

Rubber elongation factor

Small rubber particle protein

Latex native lipids

Langmuir monolayer

Polarization modulated-infrared reflection absorption spectroscopy

URI

https://oskar-bordeaux.fr/handle/20.500.12278/106530

DOI

http://dx.doi.org/10.1016/j.bbamem.2016.11.010

Project ANR

ANR-10-CD2I-08
De la particule de caoutchouc d'hévéa à la structure et aux propriétés du caoutchouc naturel: vers l'optimisation des performances du caoutchouc naturel - ANR-14-CE07-0026

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248