A rapid and reversible colorimetric assay for the characterization of aminated solid surfaces
| hal.structure.identifier | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM] | |
| dc.contributor.author | COUSSOT, Gaëlle | |
| hal.structure.identifier | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM] | |
| dc.contributor.author | PERRIN, Catherine | |
| dc.contributor.author | MOREAU, Thomas | |
| hal.structure.identifier | sSE 2011 | |
| dc.contributor.author | DOBRIJEVIC, M. | |
| hal.structure.identifier | sSE 2011 | |
| dc.contributor.author | LE POSTOLLEC, A. | |
| hal.structure.identifier | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM] | |
| dc.contributor.author | VANDENABEELE-TRAMBOUZE, Odile | |
| dc.date.issued | 2011 | |
| dc.identifier.issn | 1618-2642 | |
| dc.description.abstractEn | The covalent immobilization of synthetic or natural macromolecular compounds containing amino groups onto polystyrene (PS) solid surfaces is of great interest in diagnostic applications. A sensitive assay allowing the determination of reactive end groups is therefore a powerful tool for predicting the performance of the active surface. Recently, we reported the use of the Coomassie Brilliant Blue (CBB) colorimetric reagent to quantify protonated groups (N+) in linear and dendritic structures in solution (Coussot G et al. Polym Int 58(5):511-518). In this work, a simple method using CBB dye for the characterization of PS aminated solid-surfaces is developed. The proposed Amino Density Estimation by Colorimetric Assay (ADECA) method is based on the reversible complexation of the dye with the N+ groups on solid surfaces. The assay measures the released dye thanks to the use of a unique sodium carbonate-methanol buffer. Thereby, for the first time, the same surface can be used for characterization and for further coupling applications. A surface density of 4 N + groups per nm2 can be measured in PS microwell format, the whole characterization being done within 30 min. Performances of this new colorimetric-based method are detailed. The ADECA method is further demonstrated to be useful for the characterization of aminated polypropylene, and glass materials with various sizes and shapes. | |
| dc.language.iso | en | |
| dc.publisher | Springer Verlag | |
| dc.title.en | A rapid and reversible colorimetric assay for the characterization of aminated solid surfaces | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1007/s00216-010-4363-7 | |
| dc.subject.hal | Chimie/Chimie organique | |
| bordeaux.journal | Analytical and Bioanalytical Chemistry | |
| bordeaux.page | 1061-1069 | |
| bordeaux.volume | 399 | |
| bordeaux.issue | 3 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-00553885 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| dc.subject.it | Coomassie blue | |
| dc.subject.it | ADECA | |
| dc.subject.it | quantification | |
| dc.subject.it | amine density | |
| dc.subject.it | solid support characterization | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-00553885v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20and%20Bioanalytical%20Chemistry&rft.date=2011&rft.volume=399&rft.issue=3&rft.spage=1061-1069&rft.epage=1061-1069&rft.eissn=1618-2642&rft.issn=1618-2642&rft.au=COUSSOT,%20Ga%C3%ABlle&PERRIN,%20Catherine&MOREAU,%20Thomas&DOBRIJEVIC,%20M.&LE%20POSTOLLEC,%20A.&rft.genre=article |
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