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A rapid reversible colorimetric assay for the characterization of aminated solid surfaces

hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorCOUSSOT, Gaëlle
hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorPERRIN, Catherine
hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorMOREAU, Thomas
hal.structure.identifiersSE 2011
dc.contributor.authorDOBRIJEVIC, M.
hal.structure.identifiersSE 2011
dc.contributor.authorLE POSTOLLEC, A.
hal.structure.identifierInstitut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
dc.contributor.authorVANDENABEELE-TRAMBOUZE, Odile
dc.date.accessioned2021-07-07T16:28:12Z
dc.date.available2021-07-07T16:28:12Z
dc.date.issued2011
dc.identifier.issn1618-2642
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/96645
dc.description.abstractEnThe covalent immobilization of synthetic or natural macromolecular compounds containing amino groups onto polystyrene (PS) solid surfaces is of great interest in diagnostic applications. A sensitive assay allowing the determination of reactive end groups is therefore a powerful tool for predicting the performance of the active surface. Recently, we reported the use of the Coomassie brilliant blue (CBB) colorimetric reagent to quantify protonated groups (N +) in linear and dendritic structures in solution (Coussot et al., Polym Int 58(5):511- 518, 2009). In this work, a simple method using CBB dye for the characterization of PS aminated solid surfaces is developed. The proposed amino density estimation by colorimetric assay (ADECA) method is based on the reversible complexation of the dye with the N + groups on solid surfaces. The assay measures the released dye thanks to the use of a unique sodium carbonate-methanol buffer. Thereby, for the first time, the same surface can be used for characterization and for further coupling applications. A surface density of four N + groups per square nanometer can be measured in PS microwell format, the whole characterization being done within 30 min. Performances of this new colorimetric-based method are detailed. The ADECA method is further demonstrated to be useful for the characterization of aminated polypropylene and glass materials with various sizes and shapes.
dc.language.isoen
dc.publisherSpringer Verlag
dc.title.enA rapid reversible colorimetric assay for the characterization of aminated solid surfaces
dc.typeArticle de revue
dc.identifier.doi10.1007/s00216-010-4363-7
dc.subject.halChimie/Chimie organique
bordeaux.journalAnalytical and Bioanalytical Chemistry
bordeaux.page1061-1069
bordeaux.volume399
bordeaux.hal.laboratoriesLaboratoire d'Astrophysique de Bordeaux (LAB) - UMR 5804*
bordeaux.institutionCNRS
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00772183
hal.version1
dc.subject.itCoomassie blue . Quantification . Amine density. Solid support characterization . ADECA
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00772183v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20and%20Bioanalytical%20Chemistry&rft.date=2011&rft.volume=399&rft.spage=1061-1069&rft.epage=1061-1069&rft.eissn=1618-2642&rft.issn=1618-2642&rft.au=COUSSOT,%20Ga%C3%ABlle&PERRIN,%20Catherine&MOREAU,%20Thomas&DOBRIJEVIC,%20M.&LE%20POSTOLLEC,%20A.&rft.genre=article


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