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Anti-VEGF signalling mechanism in HUVECs by melatonin, serotonin, hydroxytyrosol and other bioactive compounds

dc.rights.licenseopenen_US
dc.relation.isnodouble722978d2-8240-44b7-ba50-158ae65410cf*
dc.relation.isnodouble6ddf6c55-c2dd-4884-ac24-1fe7dd5f14fa*
dc.contributor.authorCEREZO, Ana B.
dc.contributor.authorLABRADOR, Maria
dc.contributor.authorGUTIERREZ, Andres
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorHORNEDO ORTEGA, Ruth
dc.contributor.authorTRONCOSO, Ana M.
dc.contributor.authorGARCIA-PARRILLA, M. Carmen
dc.date.accessioned2020-07-03T09:13:01Z
dc.date.available2020-07-03T09:13:01Z
dc.date.issued2019
dc.identifier.issn2072-6643en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/8541
dc.description.abstractEnAngiogenesis drives evolution and destabilisation of atherosclerotic plaques and the growth and expansion of tumour cells. Vascular endothelial growth factor (VEGF) is the main endogenous pro-angiogenic factor in humans. The aim was to provide insight into the anti-VEGF activity of bioactive compounds derived from aromatic amino acids (serotonin, melatonin, 3-indoleacetic acid, 5-hydroxytryptophol and hydroxytyrosol). Experiments involved endothelial cell migration (wound-healing assay), the molecular mechanisms (ELISA assay) and the downstream effects (phospholipase C gamma 1 (PLCγ1), protein kinase B (Akt) and endothelial nitric oxide synthase (eNOS) by Western blot) on human umbilical vein endothelial cells (HUVECs). The data suggest for the first time that hydroxytyrosol interacts with surface components of the endothelial cell membrane (, preventing VEGF from activating its receptor. Serotonin and 5-hydroxytryptophol significantly inhibited HUVEC migration (98% and 50%, respectively) following the same mechanism. Conversely to other bioactive compounds, the anti-angiogenic effect of melatonin, serotonin, 3-indoleacetic acid and 5-hydroxytryptophol is not mediated via PLCγ1. However, hydroxytyrosol inhibits PLCγ1 phosphorylation. Additionally, melatonin and serotonin maintained eNOS phosphorylation and hydroxytyrosol significantly activated eNOS-all via Akt. These data provide new evidence supporting the interest in melatonin, serotonin, 3-indoleacetic acid, 5-hydroxytryptophol and hydroxytyrosol for their further exploitation as anti-VEGF ingredients in food.
dc.language.isoENen_US
dc.subject.en3-Indoleacetic Acid
dc.subject.en5-Hydroxytryptophol
dc.subject.enAkt
dc.subject.enPlcγ1
dc.subject.enVegf
dc.subject.enAngiogenesis
dc.subject.enEnos
dc.subject.enHydroxytyrosol
dc.subject.enMelatonin
dc.subject.enSerotonin
dc.title.enAnti-VEGF signalling mechanism in HUVECs by melatonin, serotonin, hydroxytyrosol and other bioactive compounds
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/nu11102421en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed31614459en_US
bordeaux.journalNutrientsen_US
bordeaux.page1-13en_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesOenologie - EA 4577en_US
bordeaux.issue10en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
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