FARACE, Giovanni; VISCONTI, S.; MARCHIVE, Chloé; ADUCCI, P.; MARRA, M.; TROSSAT-MAGNIN, Claudine

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Article de revue

Ce document a été publié dans

Vitis. 2015, vol. 54, n° 1, p. 33-40

Résumé en anglais

In eukaryotic cells, 14-3-3 proteins constitute a family of conserved regulatory proteins which bind to a large number of phosphorylated partners. Although a great body of evidences accumulated in herbaceous species indicates that 14-3-3s play key roles in regulation of plant growth, information about function in tree plants is still very scarce. In this paper, we report the characterization of two novel 14-3-3s from grapevine (Vitis vinifera 'Cabernet Sauvignon') named VV1_CS and VV2_CS. The VV1_CS and VV2_CS cDNA were expressed in Escherichia coli and the recombinant proteins were shown to be functional proteins using plasma membrane H+-ATPase as target. Transcript analysis during grape development or under elicitor-induced stress revealed differential expression of the two 14-3-3 isoforms.< Réduire

Mots clés

Vitis Vinifera

Cellule Eucaryote

Escherichia Coli

Cabernet Sauvignon

Saccharomyces Cerevisiae

Expression Des Gènes

Pcr

Isoforme

Mots clés en anglais

14-3-3 Proteins

Vitis Vinifera

URI

https://oskar-bordeaux.fr/handle/20.500.12278/3849

Unités de recherche

Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287