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dc.rights.licenseopenen_US
dc.contributor.authorFERNANDEZ-CRUZ, Edwin
dc.contributor.authorCEREZO, Ana B
dc.contributor.authorCANTOS-VILLAR, Emma
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorRICHARD, Tristan
dc.contributor.authorTRONCOSO, Ana M
dc.contributor.authorGARCIA-PARRILLA, M Carmen
dc.date.accessioned2021-04-09T08:08:22Z
dc.date.available2021-04-09T08:08:22Z
dc.date.issued2019-04-10
dc.identifier.issn1520-5118en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/26914
dc.description.abstractEnStilbenes are phenolic compounds present in different higher plant families that have shown different biological activities, such as antioxidant properties and antitumoral and anti-atherosclerotic effects, among others. Angiogenesis is a key process involved in both cancer and cardiovascular diseases, the vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 being the main triggers. Certain polyphenol compounds, such as flavonoids, have shown a potent capacity to inhibit VEGF and, consequently, angiogenesis. The present work, therefore, aims to evaluate the potential effect of stilbenes on inhibiting VEGF and their subsequent effect on the downstream signaling pathway (PLCγ1, Akt, and eNOS). VEGFR-2 activation was studied through an ELISA assay in the HUVEC line, while the phosphorylation of intracellular downstream proteins PLCγ1, Akt, and eNOS was tested by Western blot. Student's t test was used to determine significant differences between samples. On the one hand, astringin, pallidol, and ω-viniferin showed the lowest IC values (2.90 ± 0.27, 4.42 ± 0.67, and 6.10 ± 1.29 μM, respectively) against VEGFR-2 activation. Additionally, VEGF-induced PLCγ1 phosphorylation was significantly inhibited by ε-viniferin, astringin, and ω-viniferin. However, ε-viniferin and pallidol simultaneously enhanced eNOS activation, proving to be via Akt activation in the case of ε-viniferin. For the first time, these data suggest that stilbenes such as astringin, pallidol, ω-viniferin, and ε-viniferin have a potential anti-angiogenic effect and they could be further considered as anti-VEGF ingredients in food and beverages. In addition, ε-viniferin and pallidol significantly allowed eNOS activation and could likely prevent the side effects caused by anti-VEGF hypertension drugs.
dc.language.isoENen_US
dc.subject.enHuman Umbilical Vein Endothelial Cells
dc.subject.enHumans
dc.subject.enPhospholipase C gamma
dc.subject.enPlant Extracts
dc.subject.enProto-Oncogene Proteins c-akt
dc.subject.enSignal Transduction
dc.subject.enStilbenes
dc.subject.enVascular Endothelial Growth Factor Receptor-2
dc.subject.enVitis
dc.title.enInhibition of VEGFR-2 Phosphorylation and Effects on Downstream Signaling Pathways in Cultivated Human Endothelial Cells by Stilbenes from Vitis Spp.
dc.title.alternativeJ Agric Food Chemen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acs.jafc.9b00282en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed30892883en_US
bordeaux.journalJournal of Agricultural and Food Chemistryen_US
bordeaux.page3909-3918en_US
bordeaux.volume67en_US
bordeaux.issue14en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-03193848
hal.version1
hal.date.transferred2021-04-09T08:08:25Z
hal.exporttrue
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