For Solanaceae, the primary metabolite contents of ripening and ripe fruits have beenwidely studied in tomato. However, less data are available for other species such as pepper(Capsicum annuum L.) and eggplant (Solanum melongena L.) during fruit development tostudy their metabolism. We used 1H NMR profiling to produce quantitative metabolite dataof pepper and eggplant fruit along development.Polar compounds were extracted from lyophilized fruit powder with an ethanol-water series.NMR analyses were performed on pH-adjusted extracts containing EDTA. Metaboliteabsolute quantification was achieved using a 500-MHz-NMR-spectrometer and externalcalibration-range solutions. 1D-NMR spectra were processed with NMRProcFlow webtool(1). Annotation of the 1D-1H-spectra was performed as previously described(2,3,4),and using additional common 2D-NMR and less common 1D-selective-gradient COSY andTOCSY experiments.Twenty-four metabolites were determined in pepper (including two compounds partiallyidentified: a trans-4-hydroxyproline-like-compound, and a hydroxycinnamic-acid containingcompound)and 27 in eggplant. Nineteen common metabolites were quantified in both fruitspecies including three soluble sugars and one sugar-alcohol, five organic acids and nine freeamino acids. Spectra and data are available at recherche.data.gouv repository and ODAMdataexplorer and described in Roch et al. (2024). The metabolite quantification data allowedseeing common changes during development for the two species and their main compositionaldifferences, and also comparison with tomato data.Funding: FRIMOUSS ANR-15-CE20-0009-01, MetaboHUB ANR-11-INBS-0010References:Jacob et al. 2017, Metabolomics 13:1-5. Deborde et al. 2009, Metabolomics 5:183-198. Roch et al. 2020, J Ex Bot. 71:5823-5836. Sobolev et al. 2018, Food Chem. 255:120-131. Roch et al. 2024, BMC Research Notes 17:337.< Réduire