KYRIAKIDOU, Evangelia; CUTUGNO, Giulia; ALLARD, Camille; NADJAR, Agnes

doi:10.3791/66769

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KYRIAKIDOU, Evangelia
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

CUTUGNO, Giulia
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

ALLARD, Camille
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]

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Article de revue

Ce document a été publié dans

Journal of visualized experiments. 2024-09-06 n° 211

Résumé en anglais

Microglia, as the resident macrophages of the brain, are essential for maintaining brain homeostasis. They shape neuronal circuits during development, survey their environment for debris or dead cells, as well as respond to infection and injury in the brain, among many other functions. However, their important role in neurodevelopment and synaptic plasticity and pathophysiology has not been fully defined, highlighting the need for further investigation. To gain a more comprehensive understanding of the role of microglia in these processes, we need to isolate microglia and characterize them genetically, metabolically, and functionally. However, the isolation of microglia from adult mice, especially from small brain structures, is challenging as they represent a small percentage of the total brain cells, and the yield of isolated microglia is often too low. Here, the magnetic isolation of microglia using CD11b+ microbeads allows us to sort microglial cells from the hypothalamus of a freshly perfused adult mouse brain. The current method allows us to achieve relatively high purity and yield in a short period while maintaining cell viability.< Réduire

Mots clés en anglais

Animals

Mice

Animal

Mouse

Procedures

Metabolism

Cd11b Antigen

Microglia

Cytology

Hypothalamus

Cd11b Antigen

Immunomagnetic Separation

URI

https://oskar-bordeaux.fr/handle/20.500.12278/206893

DOI

http://dx.doi.org/10.3791/66769

Projet Européen

European Union’s Horizon 2020

Project ANR

Rôle des altérations bioénergétiques microgliales précoces dans l'hétérogénéité des phénotypes obèses dépendante du sexe - ANR-23-CE14-0076

Unités de recherche

Neurocentre Magendie - U1215