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Developing a culturomic approach to build a collection of grapevine foliar microorganisms with potential biocontrol activity against downy mildew

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorJASWA, Aarti
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
dc.contributor.authorFOURNIER, Paola
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierUtah State University [USU]
dc.contributor.authorMARTIN, Valérie
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMASNEUF-POMARÈDE, Isabelle
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARTINS, Guilherme
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorLABARTHE, Simon
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorVALLANCE, Jessica
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierBiodiversité, Gènes & Communautés [BioGeCo]
dc.contributor.authorVACHER, Corinne
dc.date.conference2024-01-15
dc.description.abstractEnA culturomic approach involves the use of a range of different culture conditions coupled with a high-throughput method of identification to be able to isolate and identify microorganisms in a sample. A number of studies have shown that using plant-based culture media rather than conventional media that contain chemically synthesized or animal-based extracts can successfully increase the cultivability of plant-associated microbiota. This study aimed to use a culturomic approach to build a collection of grapevine foliar microorganisms, which can subsequently be used to build Synthetic microbial Communities (SynComs) for biocontrol of downy mildew (caused by Plasmopara viticola). For the collection, leaves were sampled from parcels that showed high and low susceptibility to downy mildew. Alongside four conventional culture media, we developed a grapevine leaf based culture medium to cultivate epiphytic and endophytic bacteria, yeast and filamentous fungi. We proceeded to identify the isolates by MALDI-TOF MS and Sanger sequencing. Overall, we isolated 1091 microorganisms including 544 bacteria, 217 yeast and 330 filamentous fungi. We were able to identify 86% of the isolates with 93 total genera identified across the five culture media used. Using the grapevine leaf based culture medium enabled the cultivation of 4 genera of bacteria, 1 yeast genus and 13 genera of filamentous that were not seen on the other media.
dc.description.sponsorshipCultivating the grapevine without pesticides : towards agroecological wine-producing socio-ecosystems - ANR-20-PCPA-0010
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enculture collection
dc.subject.enbiocontrol
dc.subject.enCulturomics
dc.subject.enplant-based culture media
dc.subject.engrapevine microbiome
dc.subject.endowny mildew
dc.subject.enMALDI-TOF MS
dc.title.enDeveloping a culturomic approach to build a collection of grapevine foliar microorganisms with potential biocontrol activity against downy mildew
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Ecologie, Environnement
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Mycologie
dc.subject.halSciences du Vivant [q-bio]/Sciences agricoles/Sciences et techniques de l'agriculture
bordeaux.conference.titleJournées Jean Chevaugeon - 14e Rencontres de Phytopathologie - Mycologie
bordeaux.countryFR
bordeaux.conference.cityAussois
bordeaux.peerReviewedoui
hal.identifierhal-04842411
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.organizerINRAE
hal.conference.organizerL'Institut Agro Rennes-Angers
hal.conference.organizerSociété Française de Phytopathologie
hal.conference.organizerUniversité d'Angers
hal.conference.organizerCIRAD
hal.conference.organizerLabex ARBRE
hal.conference.end2024-01-19
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04842411v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=JASWA,%20Aarti&FOURNIER,%20Paola&MARTIN,%20Val%C3%A9rie&MASNEUF-POMAR%C3%88DE,%20Isabelle&MARTINS,%20Guilherme&rft.genre=conference


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