JASWA, Aarti; FOURNIER, Paola; MARTIN, Valérie; MASNEUF-POMARÈDE, Isabelle; MARTINS, Guilherme; LABARTHE, Simon; VALLANCE, Jessica; VACHER, Corinne

Métadonnées

Licence d’utilisation du document

http://creativecommons.org/licenses/by-nc-nd/

JASWA, Aarti
Santé et agroécologie du vignoble [UMR SAVE]
Unité de Recherche Œnologie [Villenave d'Ornon] [OENO]

FOURNIER, Paola
Santé et agroécologie du vignoble [UMR SAVE]
Biodiversité, Gènes & Communautés [BioGeCo]
Amélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]

MARTIN, Valérie
Santé et agroécologie du vignoble [UMR SAVE]
Utah State University [USU]

Langue

Autre communication scientifique (congrès sans actes - poster - séminaire...)

Ce document a été publié dans

Journées Jean Chevaugeon - 14e Rencontres de Phytopathologie - Mycologie, 2024-01-15, Aussois.

Résumé en anglais

A culturomic approach involves the use of a range of different culture conditions coupled with a high-throughput method of identification to be able to isolate and identify microorganisms in a sample. A number of studies have shown that using plant-based culture media rather than conventional media that contain chemically synthesized or animal-based extracts can successfully increase the cultivability of plant-associated microbiota. This study aimed to use a culturomic approach to build a collection of grapevine foliar microorganisms, which can subsequently be used to build Synthetic microbial Communities (SynComs) for biocontrol of downy mildew (caused by Plasmopara viticola). For the collection, leaves were sampled from parcels that showed high and low susceptibility to downy mildew. Alongside four conventional culture media, we developed a grapevine leaf based culture medium to cultivate epiphytic and endophytic bacteria, yeast and filamentous fungi. We proceeded to identify the isolates by MALDI-TOF MS and Sanger sequencing. Overall, we isolated 1091 microorganisms including 544 bacteria, 217 yeast and 330 filamentous fungi. We were able to identify 86% of the isolates with 93 total genera identified across the five culture media used. Using the grapevine leaf based culture medium enabled the cultivation of 4 genera of bacteria, 1 yeast genus and 13 genera of filamentous that were not seen on the other media.< Réduire

Mots clés en anglais

culture collection

biocontrol

Culturomics

plant-based culture media

grapevine microbiome

downy mildew

MALDI-TOF MS

Project ANR

Cultivating the grapevine without pesticides : towards agroecological wine-producing socio-ecosystems - ANR-20-PCPA-0010

Origine

Importé de hal

Unités de recherche

BioGeCo (Biodiversité Gènes & Communautés) - UMR 1202