CHAPARD, Christophe; MENEU, Léa; SERIZAY, Jacques; WESTBROOK, Alex; ROUTHIER, Etienne; RUAULT, Myriam; BIGNAUD, Amaury; THIERRY, Agnès; GOURGUES, Géraldine; LARTIGUE, Carole; PIAZZA, Aurèle; TADDEI, Angela; BECKOUËT, Frédéric; MOZZICONACCI, Julien; KOSZUL, Romain

doi:10.1101/2022.12.21.520625

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CHAPARD, Christophe
Régulation spatiale des Génomes - Spatial Regulation of Genomes

MENEU, Léa
Régulation spatiale des Génomes - Spatial Regulation of Genomes
Collège Doctoral

SERIZAY, Jacques
Régulation spatiale des Génomes - Spatial Regulation of Genomes

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2022-12-21

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Genomic sequences co-evolve with DNA-associated proteins to ensure the multiscale folding of long DNA molecules into functional chromosomes. In eukaryotes, different molecular complexes organize the chromosome’s hierarchical structure, ranging from nucleosomes and cohesin- mediated DNA loops to large scale chromatin compartments. To explore the relationships between the DNA sequence composition and the spontaneous loading and activity of these DNA-associated complexes in the absence of co-evolution, we characterized chromatin assembly and activity in yeast strains carrying exogenous bacterial chromosomes that diverged from eukaryotic sequences over 1.5 billion years ago. We show that nucleosome assembly, transcriptional activity, cohesin-mediated looping, and chromatin compartmentalization can occur in a bacterial chromosome with a largely divergent sequence integrated in a eukaryotic host, and that the chromatinization of bacterial chromosomes is highly correlated with their sequence composition. These results are a step forward in understanding how foreign sequences are interpreted by a host nuclear machinery during natural horizontal gene transfers, as well as in synthetic genomics projects.< Réduire

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Exogenous chromosomes reveal how sequence composition drives chromatin assembly, activity, folding and compartmentalization

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