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Separation of polyphenols by HILIC methods with diode array detection, charged aerosol detection and mass spectrometry: application to grapevine extracts rich in stilbenoids

dc.rights.licenseembargoen_US
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorGAUDIN, Karen
ORCID: 0000-0002-0755-145X
IDREF: 140101632
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorVALLS FONAYET, Josep
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCORDAZZO, Rémy
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorSERAFIN, Wiktoria
dc.contributor.authorLAFON, Emma
dc.contributor.authorGAUBERT, Alexandra
IDREF: 150487983
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorRICHARD, Tristan
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorCLUZET, Stephanie
dc.date.accessioned2024-10-09T14:30:38Z
dc.date.available2024-10-09T14:30:38Z
dc.date.issued2024-11-08
dc.identifier.issn0021-9673en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202351
dc.description.abstractEnThe characterization of plant extracts is usually accomplished by reverse-phase liquid chromatography, but the development of new complementary approaches, such as HILIC, offers an orthogonal method. In this study, five HILIC stationary phases were evaluated to assess their ability to retain polyphenols. They were selected to cover the main different HILIC mechanisms: bare silica; silica with ethylene bridge; neutral amide; amino; zwitterionic. A total of 31 polyphenol standards were used for the screening, including 9 stilbenes, 8 flavonoids, 6 anthocyanins, and 8 phenolic acids. Three different detections were tested: diode array detector, charged aerosol detector and mass spectrometry. Results indicated that silica supports were not suitable for retaining polyphenols, with no or low retention observed except for anthocyanins. The effectiveness of stationary phases in retention of phenolics following the order related to increased retention: zwitterionic, amide, and amino. The choice of mobile phase also influenced retention. Mobile phases containing TFA as pH modifier limited retention, while formic acid was found to be more effective for polyphenol retention. Ammonium buffers also improved retention but often compromised peak shape. pH changes mainly impacted ionizable compounds, such as phenolic acids, by increasing their retention when they were ionized. DAD was wellsuited for detecting polyphenols that possess aromatic rings, though peak wavelengths depend on the structures of the polyphenols. CAD, while less sensitive than DAD and MS, provided an almost similar response for structurally related compounds, even with gradient elution. MS was the preferred detector for quantification when resolution between compounds was challenging, as it is often the case with natural extracts. The study successfully demonstrated that best HILIC conditions were obtained using an amino stationary phase composed of a polyethylenimine and formic acid-based mobile phase. These conditions were successfully applied to the analysis of stilbenoid-rich extracts from different parts of the vine. The elution order of stilbenoids followed the degree of polymerization. With CAD, the chromatographic profile was more representative of sample composition. It was demonstrated for the first time the interest of a combination of HILIC and CAD for analyzing stilbenes, offering a complementary approach to the classic RP analysis.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010en_US
dc.language.isoENen_US
dc.subject.enHILIC
dc.subject.enCAD
dc.subject.enStilbene
dc.subject.enPolyethylenimine
dc.subject.enVitis vinifera
dc.title.enSeparation of polyphenols by HILIC methods with diode array detection, charged aerosol detection and mass spectrometry: application to grapevine extracts rich in stilbenoids
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.chroma.2024.465422en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalJournal of Chromatography Aen_US
bordeaux.volume1736en_US
bordeaux.hal.laboratoriesUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04728732
hal.version1
hal.date.transferred2024-10-09T14:30:42Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chromatography%20A&rft.date=2024-11-08&rft.volume=1736&rft.eissn=0021-9673&rft.issn=0021-9673&rft.au=GAUDIN,%20Karen&VALLS%20FONAYET,%20Josep&CORDAZZO,%20R%C3%A9my&SERAFIN,%20Wiktoria&LAFON,%20Emma&rft.genre=article


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