Use of the human hepcidin gene to build a positive-selection vector for periplasmic expression in Escherichia coli
Langue
en
Article de revue
Ce document a été publié dans
Analytical Biochemistry. 2016, vol. 500, p. 35-37
Elsevier Masson
Résumé en anglais
Recombinant proteins are often produced in the periplasm of Escherichia coli because this facilitates the purification process. The oxidizing environment favors the formation of disulfide bridges. We showed that the ...Lire la suite >
Mots clés en anglais
FUSION
ANTIMICROBIAL ACTIVITY
PEPTIDE
Positive selection
Hepcidin
Expression vector
Cloning
MALTOSE-BINDING PROTEIN